Changlin TIAN received his B.S. degree in biochemical engineering and applied electronic technology from Shanghai Jiaotong University in 1995, his M.S. degree in Molecular Biology from Shanghai Jiaotong University in 1997, and his Ph.D. degree in Molecular Biophysics from Florida State University/National High Magnetic Field Laboratory in 2003. Subsequently he pursued a three-year postdoc as a research fellow in biochemistry at Vanderbilt University. Following his postdoctoral work, in 2007, Changlin joined University of Science and Technology of China as a professor. In 2009-2014 and 2020-present, Changlin was appointed as vice director of High Magnetic Field Laboratory, Chinese Academy of Sciences.  

Research Interests

1. Methodological development, equipment development and application research of protein dynamic properties based on nuclear magnetic resonance (NMR), electron spin resonance (ESR), fluorescence (FLIM) and other spectroscopy methods
2. Methodological development, equipment development and basic research of "electrobiology" for biological systems based on high-field/low-temperature/time-resolved electron spin resonance (ESR)
3. Development of methods for efficient preparation of post-translationally modified proteins based on chemical total synthesis, bio-chemical semi-synthesis and other large molecular weight proteins, equipment development and research on the structure and epigenetic mechanism of nucleosomes containing post-translationally modified histones
4. Screening/modification/optimization of peptide drugs targeting ion channels and GPCR receptors, and research on the development of peptide drugs such as pharmacokinetics and pharmacokinetics

Selected Publications 

1. Li, S., F. Yang, D. Sun*, Y. Zhang, M. Zhang, S. Liu, P. Zhou, C. Shi, L. Zhang*, and C. Tian*. 2020. 'Cryo-EM structure of the hyperpolarization-activated inwardly rectifying potassium channel KAT1 from Arabidopsis', Cell Res (DOI:10.1038/s41422-020-00407-3)
2. Sun, D., S. Liu, S. Li, M. Zhang, F. Yang, M. Wen, P. Shi, T. Wang, M. Pan, S. Chang, X. Zhang, L. Zhang*, C. TIan*, and L. Liu*. 2020. 'Structural Insights into Human Acid-sensing Ion Channel 1a Inhibition by Snake Toxin Mambalgin1', Elife: e57096.
3. Huang, D. L., Y. Li, J. Liang, L. Yu, M. Xue, X. X. Cao, B. Xiao, C. L. Tian, L. Liu, and J. S. Zheng. 2020. 'The New Salicylaldehyde S,S-Propanedithioacetal Ester Enables N-to-C Sequential Native Chemical Ligation and Ser/Thr Ligation for Chemical Protein Synthesis', J Am Chem Soc, 142: 8790-99.
4. Huang, D. L., C. Montigny, Y. Zheng, V. Beswick, Y. Li, X. X. Cao, T. Barbot, C. Jaxel, J. Liang, M. Xue, C. L. Tian, N. Jamin, and J. S. Zheng. 2020. 'Chemical Synthesis of Native S-Palmitoylated Membrane Proteins through Removable- Backbone-Modification- Assisted Ser/Thr Ligation', Angew Chem Int Ed Engl, 59: 5178-84.
5. Qu, Q., S. Gao, F. Wu, M. G. Zhang, Y. Li, L. H. Zhang, D. Bierer, C. L. Tian, J. S. Zheng, and L. Liu. 2020. 'Synthesis of Disulfide Surrogate Peptides Incorporating Large-Span Surrogate Bridges Through a Native-Chemical-Ligation-Assisted Diaminodiacid Strategy', Angew Chem Int Ed Engl, 59: 6037-45.
6. Zhao, Rui, Pan Shi, Junyou Chen, Shuaishuai Sun, Jingnan Chen, Jibin Cui, Fangming Wu, Gemin Fang, Changlin Tian, Jing Shi, Donald Bierer, Lei Liu, and Yi-Ming Li. 2020. 'Chemical synthesis and biological activity of peptides incorporating an ether bridge as a surrogate for a disulfide bond', Chem Sci, 11: 7927-32.
7. Shi, P., Y. Zhang, P. Lv, W. Fang, S. Ling, X. Guo, D. Li, S. Liu, D. Sun, L. Zhang, D. Liu, J. S. Zheng, and C. Tian. 2020. 'A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET', Chem Commun (Camb), 56: 6941-44.
8. Li, J. B., J. Liang, and C. Tian. 2020. 'Chemical synthesis of di-ubiquitin modified histones for further biochemical studies', Methods Enzymol, 639: 263-87.
9. Zhang, Y., F. Yang, S. Ling, P. Lv, Y. Zhou, W. Fang, W. Sun, L. Zhang, P. Shi, and C. Tian. 2020. 'Single-particle cryo-EM structural studies of the beta2AR-Gs complex bound with a full agonist formoterol', Cell Discov, 6: 45.
10. Gong, Hongri, Yan Gao, Xiaoting Zhou, Yu Xiao, Weiwei Wang, Yanting Tang, Shan Zhou, Yuying Zhang, Wenxin Ji, Lu Yu, Changlin Tian, Sin Man Lam, Guanghou Shui, Luke W. Guddat, Luet-Lok Wong, Quan Wang, and Zihe Rao. 2020. 'Cryo-EM structure of trimeric Mycobacterium smegmatis succinate dehydrogenase with a membrane-anchor SdhF', Nat Commun, 11(1):4245.